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Illumina Cluster Station Ref. 408, DNA Cluster Generator
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By Illumina
<p>The Illumina Cluster Station Ref 408 is designed to hybridize samples onto a flow cell and amplifies them for later sequencing on a Genome Analyzer. During cluster generation, a single DNA fragment is attached to the surface of an oligonucleotide-coated flow cell and amplified to form a surface-b…
Description
<p>The Illumina Cluster Station Ref 408 is designed to hybridize samples onto a flow cell and amplifies them for later sequencing on a Genome Analyzer. During cluster generation, a single DNA fragment is attached to the surface of an oligonucleotide-coated flow cell and amplified to form a surface-bound colony. There are two main steps involved with cluster generation: cluster amplification followed by linearization, blocking, and primer hybridization. With cluster amplification the sample is hybridized onto the flow cell and then isothermally amplified. Next, the amplified sample is prepared for sequencing using the linearization, blocking, and primer hybridization processes. With the exception of preparing reagents and template DNA, all cluster generation steps are performed on the Cluster Station.
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